Protocols

Systematic establishment of gridded plots across watersheds followed by long-term monitoring of tagged individual trees for mortality and recruitment rates. Combined with environmental characterizatio...

Cytotype inference using twelve multinational microsatellites with 97% correct classification for cytotype. Individual plants defined as diploid or triploid based on maximum number of alleles observed...

Uses hyperspectral imaging (400-1000 nm) combined with Partial Least Squares Regression to correlate spectral signatures with measured leaf traits like LMA and LWC. Enables non-destructive remote meas...

PLSR modeling approach using leaf spectral reflectance measurements to extrapolate functional trait values from subset of direct measurements to larger sample sizes. Ten-fold cross-validation used for...

Standardized searches for fresh pika sign including visual and auditory detection of individuals and fresh food caches (haypiles) to determine current site occupancy. Sites without initial detection r...

Systematic point sampling along transects to quantify vegetation composition and forage quality metrics. Modified from standard methods described in Wilkening et al. 2011.

Integration of gridMET temperature data, MODIS snowmelt timing, and PARFLOW-CLM soil moisture simulations to create multi-temporal environmental predictor datasets with quantified drought metrics.

Laboratory analysis of plant samples for nitrogen content using elemental CHNSO analyzer and water content determination through oven-drying weight loss.

Airborne hyperspectral imaging (380-2500 nm) and LiDAR data collection at 1 m resolution, followed by machine learning algorithms to produce aspen cover and cytotype distribution maps.

Systematic field observation of trophic interactions between organisms, primarily plants and insects, with rotating temporal sampling across multiple sites to record feeding relationships and interact...

A custom atmospheric correction procedure for VSWIR hyperspectral data that uses ACORN-6LX software with 200-meter processing kernels incorporating local topography and view-angle geometry to account ...

A custom ray tracing algorithm that generates shade masks for hyperspectral imagery by calculating paths between the sun, ground points, and sensor using solar geometry, sensor view angles, and digita...

Unmanned aerial vehicle flights with multispectral camera to capture georeferenced imagery of forest canopy in five spectral bands, with radiometric calibration using reference panel and downwelling l...

Collection of VSWIR field spectra at leaf- and mineral-levels using an Analytical Spectral Devices FieldSpec 3 spectroradiometer with contact probe and leaf clip attachments.

Endmember spectral measurements using contact probe attachment with internal bulb illumination for close-contact surface measurements.

CNN training using image patches for pixel-based tree and shrub species classification at alpine treeline.

Laboratory technique to determine ploidy level, validated against Hordeum vulgare standards.

Collection of site spectra to validate airborne hyperspectral data acquired by NEON Aerial Observation Platform.

Collection of sun-lit leaves from trees and shrubs using slingshots or pole clippers, with samples stored in polyethylene bags with damp paper towels to prevent water loss.

Excavation of soil pits and installation of sensors at 5, 15, and 50 cm depths with proper soil horizon replacement.

Field surveys to quantify percent canopy dieback and tree mortality following severe droughts.

Direct measurement of aboveground biomass on 673 trees across five tropical countries. Compilation of tree mass dataset including 101 trees greater than 100 cm in diameter.