Protocols

Tagged individual plants are monitored twice weekly throughout the growing season to record bloom start dates, bloom end dates, and total flower production. This provides individual-level phenological...

Shade cloths suspended 3m above treatment plots accelerate snowmelt by 5-7 days when snowpack reaches ~1m depth, simulating earlier spring snowmelt conditions. Cloths removed after complete treatment ...

Weekly monitoring of alpine plant flowering phenology coupled with snowmelt date recording to assess environmental effects on plant reproductive timing. Focuses on species-specific responses to fine-s...

Statistical analysis using mixed effects models to evaluate relationships between plant clumping treatment and temperature responses while accounting for random effects of individual plants and sampli...

Long-term standardized sampling of aerial insects using a single Malaise trap at a fixed location operated weekly throughout growing seasons. Insects are collected, dried, sorted taxonomically, counte...

Controlled factorial experiment manipulating both water availability and pollen supplementation to test for interactive effects on plant reproduction.

Plant tissues are subjected to controlled freezing temperatures in a programmable chamber to determine frost damage thresholds. Temperatures are gradually reduced to simulate natural frost events, the...

Statistical analysis using zero-inflated negative binomial generalized linear mixed models to account for excess zeros in ecological count data, specifically pollen grain counts.

Linear regression analyses testing relationships between daily temperature deviations and hummingbird visitation rates. Multiple analytical approaches including yearly regressions, temperature binning...