Holshof & Swenson trait sampling protocol

Knowledge graph centered on Holshof & Swenson trait sampling protocol with 76 nodes and 144 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Barbey's Larkspur, Nuttall's Larkspur, Larkspurs.

Method synopsis

Systematic monitoring of plant disease dynamics across elevation gradient to capture environmental variation effects. Involves establishment of standardized transects with complete plant population mapping and continuous weather monitoring.

Synthesized from method descriptions across 22 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — A pathogen's spatial range is not constrained by geographical features in the flax rust pathosystem (2023), Ecology and Evolution. The protocol was originally introduced by A comparative study of plant species diversity on three historic mining sites (2011). Implementations in other papers (listed below) may differ.

1. Select elevation transects
   Twenty-three total elevation transects were systematically selected from the Upper Gunnison Basin's established hiking trails to cover the diversity of land cover and topography of the region. Trails that spanned large elevation gains and losses and trails that captured significant variation in slope aspect and grade were prioritized.
   Quantities: 23 transects total across the Upper Gunnison Basin areaDuration: Selection completed prior to 2021 field seasonConditions: Transects selected to maximize geographical and environmental variation
   Equipment: GPS, established hiking trails
2. Traverse transects on foot
   Twenty-three total elevation transects were traversed by foot. For each transect, the coordinates of the transect line and the precise latitudes and longitudes bounding every flax population were recorded.
   Quantities: 23 transects traversed, with coordinates recorded for each transect line and all flax population boundariesDuration: Conducted over the course of 2021 summer field seasonConditions: Field surveys conducted during 2021 summer growing season
   Equipment: GPS units for coordinate recording
3. Identify flax populations

Typical Equipment

• Garmin GPS units
• environmental sensors
• temperature loggers
• humidity loggers
• rain gauges
• wind speed sensors
• measuring tapes

Output Measurements

• plant population maps
• infection status
• weather time series
• elevation gradients

Papers Using This Protocol (22)

Species Studied With This Protocol

Places Where Used