Protocols

Measurement of plant species-specific cover, identification of focal species, and collection of plant biomass and functional traits including specific leaf area and leaf dry matter content.

Continuous measurement of soil CO2 concentrations at multiple depths using automated sensors to calculate soil CO2 fluxes via gradient approach. Includes soil temperature and moisture monitoring for e...

Comprehensive measurement of vegetative traits including specific leaf area, trichome density, water use efficiency, and percent water content from field-collected leaves using scanning, drying, weigh...

A 2×2 factorial experiment combining passive warming using open-top chambers with dominant plant species removal across elevational gradients at multiple global mountain sites to test interactive effe...

Factorial experiment crossing nitrogen addition treatments with dominant species removal treatments in replicated plots to test community responses to multiple global change drivers. Includes both sel...

Multivariate analysis technique to quantify community responses over time. Treatment weights indicate response magnitude and species weights show individual taxa responses.

Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.

Standardized protocol for determining dry mass of plant tissues through controlled oven drying at 60°C for 48 hours followed by precision weighing to calculate dry matter content and mass ratios.

A comprehensive systematic review using database searches, citation network expansion, and vote counting to assess evidence for macroecological rules in microorganisms across multiple taxonomic groups...

Measurement of six different soil extracellular enzyme activities using microplate spectrophotometry to understand microbial activity and nutrient cycling processes in response to plant removal treatm...