Filtered by neighborhood:Alpine Plant and Pollinator Demography Under Climate Change Clear filter

Protocols

16 protocols

mark-recapture

Beetles were captured using speed nets, marked with tungsten needle dot codes on elytra, and released for recapture monitoring to estimate population sizes and dispersal patterns. Elytral condition wa...

demographic monitoring43 mentionslong handled speed net, plastic containers for transport...

analyticalstandardized

Mantel test (Plantae)

Meta-analytical approach to synthesize population-level climate sensitivities across multiple species using structured population models and standardized effect sizes. Incorporates life history traits...

meta-analysis32 mentionsLiterature databases, Web of Science search...

observationalstandardized

NOAA weather station monitoring

Long-term systematic collection of first sighting and median activity dates for plants, insects, mammals, amphibians and birds in a high-elevation montane ecosystem. Standardized protocols track flowe...

phenological monitoring16 mentionssystematic observation protocols

analyticalstandardized

Carbon isotope discrimination analysis

Measurement of water-use efficiency differences between plant sexes using leaf carbon isotope ratios (δ13C) to understand mechanistic basis of sex-specific climate responses.

physiological measurement13 mentionswater potential measurement device, stomatal conductance measurement device...

computationalstandardized

Integral Projection Model

Size-structured population model that incorporates climate effects on vital rates to project population dynamics and calculate population growth rates. Uses continuous size distributions and matrix pr...

population modeling22 mentionsstatistical software for GLMM fitting, R software...

sampling

Gynodioecious plant pollen receipt census

Field census method to quantify pollen receipt patterns in populations of gynodioecious plants with varying sex ratios and floral densities. Involves systematic counting of flowers by sex and measurem...

reproductive ecology monitoring7 mentionsfield counting methods, pollen counting methods...

experimental

Colony fragmentation and diet manipulation of Formica podzolica (Formicidae)

Wild ant colonies were collected and split into paired fragments, then maintained on controlled diets (carbohydrate vs protein-rich) to test effects of macronutrient balance on behavior. Colony fragme...

behavioral manipulation2 mentions15-litre plastic containers, 19-litre plastic buckets...

sampling

High-precision GPS population density mapping (Plantae)

Uses centimeter-precision GPS to map exact locations of all individuals in study plots, enabling calculation of population density and neighbor relationships. Plot sizes are adjusted to population cha...

spatial mapping8 mentionsEmlid Reach RS2 GNSS receiver

measurementstandardized

Ovary response measurement for fertilization assessment

Post-pollination measurement of ovary size changes as a proxy for detecting fertilization events and ovule development. Uses standardized photography and image analysis to quantify ovary dimensions.

reproductive biology3 mentionsdissecting microscope, Leica camera...

analyticalstandardized

climatic window analysis

Statistical approach using the climwin package to determine optimal phenological windows of climate variables that best predict biological timing by testing multiple window lengths and start dates.

phenological2 mentionsclimwin R package

experimental

hand-pollination crossing (Orobanchaceae)

Controlled laboratory assay using prepared stigmas on growth medium to test pollen germination and tube growth across species boundaries. Stigmas are prepared from unopened buds, treated with pure pol...

reproductive biology3 mentionspaintbrush, dissection tools...

computational

Climate wetness index calculation

Calculation of site-specific wetness indices using mean annual precipitation and temperature data to characterize local climate conditions for experimental sites.

environmental characterization2 mentionsClimate databases, GIS software

experimental

clay caterpillar predation bioassay

Artificial prey made from green modeling clay are deployed on tree branches to estimate bird attack rates over a standardized time period. Attack marks on clay allow identification of predator type an...

predation assay2 mentionsClay extruder (Walnut Hollow), Green nontoxic modeling clay (Sargent Art)...

observational

eBird citizen science protocol

Use existing citizen science bird observation data from eBird database to characterize bird abundance and diversity patterns across environmental gradients. Data is filtered for quality and geographic...

community survey2 mentionseBird database, R package Elevatr (Hollister et al., 2021)...

analytical

Transient LTRE analysis

Analysis method that reveals demographic and trait mediated processes that buffer population growth using transient life table response experiments.

2 mentions

analytical

Water use efficiency analysis (Plantae)

R script analysis of sex differences in integrated water use efficiency and population operational sex ratios in Valeriana edulis.

3 mentions